Analytical Ultracentrifugation V [electronic resource]

Analytical Ultracentrifugation V
Author: Helmut Cölfen
Published by Springer Berlin Heidelberg
ISBN: 978-3-540-66175-7
DOI: 10.1007/3-540-48703-4

Table of Contents:

• The installation of an eight-cell Schlieren optics multiplexer in a Beckman Optima XLI/XL analytical ultracentrifuge used to measure steep refractive index gradients
• New facilities to improve the properties of Schlieren optics
• Shift interferometry in analytical ultracentrifugation of polymer solutions
• Investigation of the efficiencies of stabilizers for nanoparticles by synthetic boundary crystallization ultracentrifugation
• A comparison of evaluation methods for sedimentation velocity data of polymer solutions
• Determination of the particle size distribution of 5–100-nm nanoparticles with the analytical ultracentrifuge: consideration and correction of diffusion effects
• Pseudo synthetic boundary experiments: a new approach to the determination of diffusion coefficients from sedimentation velocity experiments
• Application of the solvent density variation method to sedimentation velocity experiments on biological systems
• Hydrostatic pressure in ultracentrifugation
• The correct analysis of low-speed sedimentation equilibrium distributions recorded by the Rayleigh interference optical system in a Beckman XL-I ultracentrifuge
• Allowance for thermodynamic nonideality in the analysis of sedimentation equilibrium distributions reflecting complex formation between dissimilar reactants
• Normalized scaling relations as a natural classification of linear macromolecules according to size
• Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules
• Calculation of structural parameters from hydrodynamic data
• Prediction of hydrodynamic parameters from 3D structures
• Solution properties of supramolecular cobalt coordination arrays
• Working with multidetection in the analytical ultracentrifuge: the benefits of the combination of a refractive index detector and an absorption detector for the analysis of colloidal systems
• Sedimentation velocity analysis of oligomeric enzymes in hydrated reversed micelles of surfactants in organic solvents
• Spectroscopic and hydrodynamic investigations of nonionic and zwitterionic detergents
• Microemulsion polymerization of butyl acrylate and methyl methacrylate

Technical and methodological innovations -- The installation of an eight-cell Schlieren optics multiplexer in a Beckman Optima XLI/XL analytical ultracentrifuge used to measure steep refractive index gradients -- New facilities to improve the properties of Schlieren optics -- Shift interferometry in analytical ultracentrifugation of polymer solutions -- Investigation of the efficiencies of stabilizers for nanoparticles by synthetic boundary crystallization ultracentrifugation -- Innovations in data analysis -- A comparison of evaluation methods for sedimentation velocity data of polymer solutions -- Determination of the particle size distribution of 5–100-nm nanoparticles with the analytical ultracentrifuge: consideration and correction of diffusion effects -- Pseudo synthetic boundary experiments: a new approach to the determination of diffusion coefficients from sedimentation velocity experiments --^
Application of the solvent density variation method to sedimentation velocity experiments on biological systems -- Hydrostatic pressure in ultracentrifugation -- The correct analysis of low-speed sedimentation equilibrium distributions recorded by the Rayleigh interference optical system in a Beckman XL-I ultracentrifuge -- Allowance for thermodynamic nonideality in the analysis of sedimentation equilibrium distributions reflecting complex formation between dissimilar reactants -- Hydrodynamics/Modeling -- Normalized scaling relations as a natural classification of linear macromolecules according to size -- Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules -- Calculation of structural parameters from hydrodynamic data -- Prediction of hydrodynamic parameters from 3D structures -- Synthetic polymers, colloids and supramolecular systems -- Solution properties of supramolecular cobalt coordination arrays --^
Working with multidetection in the analytical ultracentrifuge: the benefits of the combination of a refractive index detector and an absorption detector for the analysis of colloidal systems -- Sedimentation velocity analysis of oligomeric enzymes in hydrated reversed micelles of surfactants in organic solvents -- Spectroscopic and hydrodynamic investigations of nonionic and zwitterionic detergents -- Microemulsion polymerization of butyl acrylate and methyl methacrylate -- Sedimentation, translational diffusion, and viscosity of lactosylated polyamidoamine dendrimers -- Biological systems -- A conformation spectrum analysis of the morphological states of myosin S1 in the presence of effectors -- Combining ultracentrifugation with fluorescence to follow the unfolding of modules 16-17 of complement receptor type 1 -- Determination of partial molal volumes, and salt and water binding, of highly charged biological macromolecules (tRNA, halophilic protein) in multimolar salt solutions --^
Studying membrane proteins in detergent solution by analytical ultracentrifugation: different methods for density matching -- Analytical ultracentrifugation of the nitrogenase of Azotobacter vinelandii under anaerobic conditions -- Combining sedimentation velocity with SEC-MALLS to probe the molecular structure of heterogeneous macromolecular systems that cannot be preparatively separated: application to three rice starches -- Inhomogeneity and conformational parameters of low-substituted carboxymethyl cellulose from analytical ultracentrifugation data -- UV tagging leaves the structural integrity of an arabino-(4-O-methylglucurono)xylan polysaccharide unaffected -- Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide -- Interacting systems and assemblies -- Towards deconvoluting the interaction of the Bacillus subtilis sporulation proteins SinR and SinI using tryptophan analogue incorporated proteins --^
Use of the tryptophan analogue 7-azatryptophan to study the interaction of ? N with Escherichia coli RNA polymerase core enzyme -- Nonequilibrium self-association of a cpn60 chaperonin induced by tryptophan mutation
The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including: Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies. In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare. Therefore, this volume will be a helpful source of information to anyone who wants to catch up with the most recent developments and results related to this important analytical method